P-102: Effect of Vitrification in Open Pulled Straw Vitrification of Live Birth Rates, Fertility and The Development of Mouse Oocytes at Metaphase II

Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of HMG and HCG, mature mouse oocyte were delivered from fallopian tube.800 mature oocyte were allocated in tree groups, 250 for verification in conventional straw(Cs),280 for verification in open pulled straw (ops), 270 as control group. Results: Survival rate of oocyte in Cs was significantly lower than ops (p=0.01). After insemination, fertilization rate in oocyte was in Cs was significantly smaller than ops (p=0.001). There is significant difference in fertilization rate between ops and control group (p=0.031). After culturing, developmental capability in oocyte that were treated in ops significantly over than of oocyte who were treated in Cs (p=0.001) Conclusion: Ops technology has provide better results from point of view of survival rate, fertilization and developmental capability than that’s of Cs but use of this method as usual in IVF clinics remains collusive.